Journal: Gastroenterology

Article Title: Lamin A/C Maintains Exocrine Pancreas Homeostasis by Regulating Stability of RB and Activity of E2F

doi: 10.1053/j.gastro.2018.01.024

Figure Lengend Snippet: (A) RB protein is decreased in LMNA-KO pancreata (4d). E2F target-transcripts were quantified using RT-qPCR (6d). N=6 mice/group. (B) Immunofluorescence of pancreata (6d) from panel-A. Solid arrows: PCNA+/LMNA- cells. Dashed arrow: PCNA+/LMNA+ cells. Bar=20μm. (C) Pancreata (21d) were stained as indicated. Arrows highlight positive staining. Bar=50μm. Histograms: Quantification of Sirius-Red, MPO-activity and DBA-staining. Wild-type mice (FVB background), injected with saline (Con) or cerulein (Cer), were used as controls for the MPO assay. (D) Relative pancreatic mass (N=13-15 mice/group) and mRNA expression of fibrosis markers determined by RT-qPCR (N=8 mice/group). (E) Immunoblot of pancreata lysates (21d) from control/KO mice (4 pancreata/genotype). (F) Proposed model of LMNA role in pancreatic homeostasis. (*p≤0.05,**p≤0.01,***p≤0.001).

Article Snippet: The antibodies used included (company/source, target protein, clone/catalog number, application (WB, IF, IHC)): BD Biosciences, CD45, 30-F11, IHC; Cell Signaling Technology, Atf2, 20F1, WB; BiP/Grp78, PA5, WB; Casp7, 9492S, WB; cCasp3, ASP175.IHC; cCasp7, D198, WB; Chop, C50B12, WB; LMNA, 4C11, IF; PCNA, D3H8P, IF, IHC; PDI, C81H6, WB; LC3B, 2775, WB; Developmental Studies Hybridoma Bank, K19, Troma 3, WB; RB, 4.1C, WB; Neomarkers Inc, Hsp60, LK2, WB; Hsp70, W27, WB; Santa Cruz Biotechnology, Atf4, C-20, WB; LMNA, H-110, WB, IF; Lipase, A-3, WB; Sigma-Aldrich, Vimentin, 13.2, WB; ACTA, A5228, WB; Amylase, A8273, WB, IF; Thermo Scientific, ACTA, 710487, IHC.

Techniques: Quantitative RT-PCR, Immunofluorescence, Staining, Activity Assay, Injection, Saline, MPO Assay, Expressing, Western Blot, Control

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

doi: 10.1007/s00018-025-06071-3

Figure Lengend Snippet: Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and cyclin D in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Article Snippet: The antibody against CEBPB (PB9171, BA0670, 1:500, Boster, Wuhan, China), H3K27ac (A7253, 1:500, ABclonal, Wuhan, China), H3K4me1 (A2355, 1:500, Wuhan, China), PCNA (A00125, 1:500, Boster, Wuhan, China), Cyclin A (PB0515, 1:500, Boster, Wuhan, China), Cyclin D (BM4272, 1:500, Boster, Wuhan, China), IL-6 (AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000, Cell Signaling, MA, US), HSPA9 (14887-1-AP, 1:5000, Proteintech, IL, USA), VDAC1 (10866-1-AP, 1:5000, Proteintech, IL, USA), and β-actin (TA-09, 1:1000, ZSGB‐BIO, Beijing, China) was incubated at 4 °C overnight, followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h, and proteins were visualized with enhanced chemiluminescence reagents.

Techniques: Western Blot, Knockdown, Negative Control

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate

doi: 10.1007/s00018-025-06071-3

Figure Lengend Snippet: Silencing of LINC01013 inhibited hypoxia-induced hPASMC proliferation and inflammation. A , B hPASMC proliferation was determined by CCK8 and EdU incorporation assays ( n = 6). EdU (red), DAPI (blue), scale bar, 50 μm. C Western blotting analysis of PCNA, cyclin A and cyclin D in hPASMCs ( n = 6). D RT‒qPCR analysis showed the mRNA levels of TNF-α and IL-6 after LINC01013 knockdown, β-actin was used as a internal reference gene ( n = 6). E Western blotting analysis of TNF-α and IL-6 in hPASMCs. ( n = 6). All values are presented as the mean ± SEM. Statistical analysis was performed with one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001. Nor, normoxia; Hyp, hypoxia; NC, negative control; si, siRNA

Article Snippet: The antibody against CEBPB (PB9171, BA0670, 1:500, Boster, Wuhan, China), H3K27ac (A7253, 1:500, ABclonal, Wuhan, China), H3K4me1 (A2355, 1:500, Wuhan, China), PCNA (A00125, 1:500, Boster, Wuhan, China), Cyclin A (PB0515, 1:500, Boster, Wuhan, China), Cyclin D (BM4272, 1:500, Boster, Wuhan, China), IL-6 (AF7236, 1:500, Beyotime, Shanghai, China), TNF-α (AF8208, 1:500, Beyotime, Shanghai, China), PKM2 (4053, 1:1000, Cell Signaling, MA, US), HK II (66974-1-Ig, 1:1000, Proteintech, IL, USA), PDH (2784, 1:1000, Cell Signaling, MA, US), HSPA9 (14887-1-AP, 1:5000, Proteintech, IL, USA), VDAC1 (10866-1-AP, 1:5000, Proteintech, IL, USA), and β-actin (TA-09, 1:1000, ZSGB‐BIO, Beijing, China) was incubated at 4 °C overnight, followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies at room temperature for 1 h, and proteins were visualized with enhanced chemiluminescence reagents.

Techniques: Western Blot, Knockdown, Negative Control